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cell culture human non small cell lung cancer cell lines  (ATCC)


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    ATCC cell culture human non small cell lung cancer cell lines
    Cell Culture Human Non Small Cell Lung Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9086 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 9086 article reviews
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    Cell Culture:

    Article Title: FS536, a novel nitric oxide-releasing doxorubicin hybrid, reverts multidrug resistance in lung cancer cells.
    Article Snippet: .. Cell culture Human non-small cell lung cancer A549 cells and normal human bronchial epithelium (BEAS-2B) (ATCC; Manassas, VA, USA) were cultured in RPMI-1640 supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, 0.5% ciproxin, 2 mmol/L L-asparagine, 2 mmol/L L-arginine, 2 mmol/L Lglutamine and 10 mM of HEPES buffer (all from Gibco; New York, NY, USA). ..

    Article Title: Non-Small Cell Lung Cancer A549 cells induces HUVECs proliferation and migration through TRPV3 promoting the secretion of VEGF
    Article Snippet: .. Methods Cell Culture Human non-small cell lung cancer A549 cells were acquired from the American Type Culture Collection. .. A549 cells were cultured in Dulbecco’s modi ed Eagle’s medium (DMEM) (HyClone) containing 10% fetal bovine serum (FBS) (HyClone) in the presence of 100 IU/mL penicillin (Sigma) and 100 μg/mL streptomycin (Sigma).



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    Fig. 3 The cytotoxicity of CDDO-Me on different NSCLC cells. (a) Schematic representation of CDDO-Me identification. (b) H1975, PC9-ER, <t>A549</t> and HEK 293T cells were treated with CDDO-Me (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 5, 10, 15 and 20 µM) for 48 h, respectively. Cell viability was examined by CCK-8 assay and shown as relative viability compared to the untreated control. Each test was performed in triplicate. (c) The IC50 values of CDDO-Me in H1975, PC9- ER, A549 and HEK 293T cells were presented as the mean ± SD. (d) Colony formation of H1975, PC9-ER, and A549 cells were measured after CDDO-Me (0, 0.2, 0.4, and 0.6 µM) treatment for 14 days, and photographs of crystal violet-stained colonies were depicted. (e) The statistical result of colony number after CDDO-Me treatment. Data are shown as mean ± S.D., n = 3. One-way ANOVA with Tukey’s multiple-comparisons test, NS no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    Fig. 3 The cytotoxicity of CDDO-Me on different NSCLC cells. (a) Schematic representation of CDDO-Me identification. (b) H1975, PC9-ER, <t>A549</t> and HEK 293T cells were treated with CDDO-Me (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 5, 10, 15 and 20 µM) for 48 h, respectively. Cell viability was examined by CCK-8 assay and shown as relative viability compared to the untreated control. Each test was performed in triplicate. (c) The IC50 values of CDDO-Me in H1975, PC9- ER, A549 and HEK 293T cells were presented as the mean ± SD. (d) Colony formation of H1975, PC9-ER, and A549 cells were measured after CDDO-Me (0, 0.2, 0.4, and 0.6 µM) treatment for 14 days, and photographs of crystal violet-stained colonies were depicted. (e) The statistical result of colony number after CDDO-Me treatment. Data are shown as mean ± S.D., n = 3. One-way ANOVA with Tukey’s multiple-comparisons test, NS no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    Fig. 3 The cytotoxicity of CDDO-Me on different NSCLC cells. (a) Schematic representation of CDDO-Me identification. (b) H1975, PC9-ER, <t>A549</t> and HEK 293T cells were treated with CDDO-Me (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 5, 10, 15 and 20 µM) for 48 h, respectively. Cell viability was examined by CCK-8 assay and shown as relative viability compared to the untreated control. Each test was performed in triplicate. (c) The IC50 values of CDDO-Me in H1975, PC9- ER, A549 and HEK 293T cells were presented as the mean ± SD. (d) Colony formation of H1975, PC9-ER, and A549 cells were measured after CDDO-Me (0, 0.2, 0.4, and 0.6 µM) treatment for 14 days, and photographs of crystal violet-stained colonies were depicted. (e) The statistical result of colony number after CDDO-Me treatment. Data are shown as mean ± S.D., n = 3. One-way ANOVA with Tukey’s multiple-comparisons test, NS no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    Fig. 3 The cytotoxicity of CDDO-Me on different NSCLC cells. (a) Schematic representation of CDDO-Me identification. (b) H1975, PC9-ER, <t>A549</t> and HEK 293T cells were treated with CDDO-Me (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 5, 10, 15 and 20 µM) for 48 h, respectively. Cell viability was examined by CCK-8 assay and shown as relative viability compared to the untreated control. Each test was performed in triplicate. (c) The IC50 values of CDDO-Me in H1975, PC9- ER, A549 and HEK 293T cells were presented as the mean ± SD. (d) Colony formation of H1975, PC9-ER, and A549 cells were measured after CDDO-Me (0, 0.2, 0.4, and 0.6 µM) treatment for 14 days, and photographs of crystal violet-stained colonies were depicted. (e) The statistical result of colony number after CDDO-Me treatment. Data are shown as mean ± S.D., n = 3. One-way ANOVA with Tukey’s multiple-comparisons test, NS no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    cell culture human non small cell lung cancer a549  (ATCC)
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    Fig. 3 The cytotoxicity of CDDO-Me on different NSCLC cells. (a) Schematic representation of CDDO-Me identification. (b) H1975, PC9-ER, <t>A549</t> and HEK 293T cells were treated with CDDO-Me (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 5, 10, 15 and 20 µM) for 48 h, respectively. Cell viability was examined by CCK-8 assay and shown as relative viability compared to the untreated control. Each test was performed in triplicate. (c) The IC50 values of CDDO-Me in H1975, PC9- ER, A549 and HEK 293T cells were presented as the mean ± SD. (d) Colony formation of H1975, PC9-ER, and A549 cells were measured after CDDO-Me (0, 0.2, 0.4, and 0.6 µM) treatment for 14 days, and photographs of crystal violet-stained colonies were depicted. (e) The statistical result of colony number after CDDO-Me treatment. Data are shown as mean ± S.D., n = 3. One-way ANOVA with Tukey’s multiple-comparisons test, NS no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    Fig. 3 The cytotoxicity of CDDO-Me on different NSCLC cells. (a) Schematic representation of CDDO-Me identification. (b) H1975, PC9-ER, A549 and HEK 293T cells were treated with CDDO-Me (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 5, 10, 15 and 20 µM) for 48 h, respectively. Cell viability was examined by CCK-8 assay and shown as relative viability compared to the untreated control. Each test was performed in triplicate. (c) The IC50 values of CDDO-Me in H1975, PC9- ER, A549 and HEK 293T cells were presented as the mean ± SD. (d) Colony formation of H1975, PC9-ER, and A549 cells were measured after CDDO-Me (0, 0.2, 0.4, and 0.6 µM) treatment for 14 days, and photographs of crystal violet-stained colonies were depicted. (e) The statistical result of colony number after CDDO-Me treatment. Data are shown as mean ± S.D., n = 3. One-way ANOVA with Tukey’s multiple-comparisons test, NS no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Journal: Cell communication and signaling : CCS

    Article Title: Machine learning-aided discovery of T790M-mutant EGFR inhibitor CDDO-Me effectively suppresses non-small cell lung cancer growth.

    doi: 10.1186/s12964-024-01954-7

    Figure Lengend Snippet: Fig. 3 The cytotoxicity of CDDO-Me on different NSCLC cells. (a) Schematic representation of CDDO-Me identification. (b) H1975, PC9-ER, A549 and HEK 293T cells were treated with CDDO-Me (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 5, 10, 15 and 20 µM) for 48 h, respectively. Cell viability was examined by CCK-8 assay and shown as relative viability compared to the untreated control. Each test was performed in triplicate. (c) The IC50 values of CDDO-Me in H1975, PC9- ER, A549 and HEK 293T cells were presented as the mean ± SD. (d) Colony formation of H1975, PC9-ER, and A549 cells were measured after CDDO-Me (0, 0.2, 0.4, and 0.6 µM) treatment for 14 days, and photographs of crystal violet-stained colonies were depicted. (e) The statistical result of colony number after CDDO-Me treatment. Data are shown as mean ± S.D., n = 3. One-way ANOVA with Tukey’s multiple-comparisons test, NS no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Article Snippet: Cell lines and cell culture Human non-small cell lung cancer cell lines A549 and H1975 were purchased from the American Type Culture Collection (ATCC, CCL-185, CRL-5908), PC9 cell line was purchased from European Collection of Authenticated Cell Cultures (ECACC, 90071810), HEK 293T cell line was purchased from the American Type Culture Collection (ATCC, CRL-3216).

    Techniques: CCK-8 Assay, Control, Staining